Course Description


There will be 2–3 lectures each day throughout. Lectures will cover topics from basic optics, detectors and light sources through to photochemistry involved in the development of novel fluorescent probes and the application of advanced super-resolution optical microscopy techniques. The first 3 days will be spent setting up microscopes for brightfield and epifluorescence microscopy, including polarisation and interference contrast methods. The final 6 days will be organised in rotations around the 9 experiments listed below. The experiments will be in the large classroom at the MBA and in rooms nearby. Use will be made of marine specimens where possible and students may have an opportunity to trawl for benthos and plankton, depending on weather and availability of the MBA research vessel.

Experiments with confocal imaging. CCD imaging and deconvolution, microinjection, genetically encoded probes.

Confocal imaging with electrophysiology
CCD imaging with electrophysiology
Photolysis and photometry
Total internal reflection fluorescence imaging
Laser traps
Marine bioluminescence

Special demonstrations and hands-on exercises

Multi-photon and confocal imaging
Quantitative interference microscopy
Use of an optical bench for basic experiments on scanning and apertures
Spectroscopy and filters
Abbe’s experiments on the role of diffraction in microscope image formation with absorption and phase objects.

  • Start Date: 2021-03-17

  • End Date: 2021-03-27

  • Taught annually