Course Description

With the advent of the digital era, nowadays images are multidimensional numerical data that can be processed and analyzed quantitatively to extract more information. With this course, we intend to give a general introduction to ImageJ/Fiji (, show how to use it to extract quantitative data, and how to write simple macros.


Course approximate duration: 20 hours (12th October afternoon, 13th October full day, 14th October full day, 15th October morning)
Target audience: All scientific community with interest in the analysis of light microscopy images.
Requirements: Laptop with Image/Fiji installed. No previous experience in the analysis of light microscopy images is required.

Course organizers: Mafalda Sousa, Advanced Light Microscopy, i3S and Paula Sampaio, Advanced Light Microscopy, i3S


Porto, Portugal

  • Start Date: 2021-10-12
  • End Date: 2021-10-15