Multi-dimensional live and fixed microscope data can be collected in many ways and with a wide range of imaging modalities to help advance cellular, animal and large sample/cleared sample imaging to the next level. Currently, many courses focus on general optical principles and the use of conventional microscope platforms, such as widefield fluorescence, confocal and multiphoton imaging. However, none of these courses are specifically designed to demystify rapidly evolving and increasingly prescient methods such as light-sheet imaging.