Multiphoton microscopy has been established as the 3D imaging method of choice for studying biomedical specimens, from single cells to whole animals, with sub-micron resolution during 1990. Three decades have passed since the realization of two-photon microscopy, and the ever-expanding scope of applications and continuing instrumental innovations require a forum where new ideas can be exchanged and presented. We started the multiphoton conference in 2001 to address this need. Our conference in the SPIE BiOS 2022 meeting continues to focus on the new developments in multiphoton microscopy.
Submitted proceedings manuscripts may also be submitted to the Journal of Biomedical Optics (please visit http://spie.org/jbo for details).
Topics include:
multiphoton (one- or two- or three- or more photons) microscopy theory and system development
multiphoton (MP) in wide-field and laser scanning confocal microscopy
infrared lasers for MP systems (femtosecond vs. picosecond vs. CW)
tissue engineering, endoscopy and intravital or in vivo imaging using multiphoton microscopy
endogenous molecular (NADH/FAD/Tryptophan) imaging, energy metabolism and its applications
harmonic generation microscopy (SHG, THG) and its applications
two-photon light sheet microscopy
fluorescence resonance energy transfer imaging (FRET) and its applications
fluorescence lifetime imaging (frequency and time domain) (FLIM) and its applications
fluorescence correlation spectroscopy and image cross correlation spectroscopy (FCS, ICCS)
fluorescence recovery after photobleaching (FRAP), uncaging and photoactivation
various biological applications including developmental, neurobiology, plant biology, calcium, and pH imaging
photo-thermal, -chemical and -mechanical effects of IR radiation
laser safety and other related applications.
