The Spatial Analysis for Biological Imaging workshop is a two-day workshop to be held from Wednesday, March 29, 2023 to Thursday, March 30, 2023 at the Department of Mathematics, Imperial College London, South Kensington Campus, London. The following invited speakers have been confirmed for this workshop: Carolina Wählby (Uppsala University). Dylan Owen (University of Birmingham), Thibault Lagache (Institut Pasteur), Anthea Monod (Imperial College London), Anca Grapa (Institute of Cancer Research, London).