The Advanced Microscopy Fellows at Harvard Medical School learn quantitative microscopy techniques including confocal, TIRF, FRET, FRAP, photo-activation, single-molecule imaging, light sheet and super-resolution microscopy, and key skills needed to manage a large, heavily-used core facility. Fellows learn to train core facility users to select and apply the appropriate techniques, using a wide range of biological specimens and experimental approaches. Fellows also have the opportunity to pursue additional scholarly interests, such as: organizing and teaching microscopy lectures and workshops; organizing microscopy discussion groups/journal clubs; troubleshooting equipment problems; developing protocols for testing equipment performance; designing and/or implementing novel or custom imaging techniques.