We provide members of the University and their visitors with a wide spectrum of microscopy equipment and image analysis software packages, together with extensive training in their use. Systems include state-of-the-art optical imaging using widefield, confocal, multiphoton, light-sheet and super-resolution microscopy. We also provide consultation on sample preparation and immunolabeling procedures. We train researchers to use the microscopes and image acquisition software themselves, providing assistance when necessary to ensure collection of high quality images. Staff also conduct more complex work on a collaborative basis with the scientists.
The center currently houses several widefield fluorescence/transmitted light microscopes (Zeiss and Olympus), two DeltaVision Image Restoration microscopes (API/GE Healthcare), three laser scanning confocal microscopes (a Zeiss LSM 780, a Leica SP8 and a Zeiss LSM 880 NLO system), a Yokagawa spinning disk confocal microscope (Zeiss/Spectral Applied), an FV1000MPE Twin upright multiphoton system (Olympus), a multi-line STORM/TIRF system (Nikon) fitted with widefield-FLIM and TIRF-FLIM capabilities (Lambert Instruments), an LCV110 "VivaView" Incubator Microscope (Olympus), a Yokawawa CV1000 "CellVoyager" environmental spinning disk system (Olympus), an Ultramicroscope light sheet system (LaVision BioTec), a laser microdissection system (MMI), and an OMX 3D-SIM super-resolution microscope (API/GE Healthcare). Most of the systems are fitted with environmental chambers for live cell imaging.
The center has five full-time research support staff including the director. The center is staffed during business hours and under an open-access model, trained investigators can use the facility 24/7. In addition, many of the systems can be operated remotely via the center's on-line facility management software.
Funded initially by a five-year Beckman Foundation grant to the Rockefeller University, the Optical Engineer will contribute to the development of a new, integrated platform for the acquisition and analysis of data from pre-commercial light-sheet instruments for microscopy on living organisms and cells. The Optical Engineer will work in close collaboration with optical microscopy and image analysis staff, biologists, physicists, bioinformaticians, computer scientists, and precision instrumentation fabrication experts in an international and interdisciplinary environment. Responsibilities include maintaining and modifying pre-commercial light sheet microscopes as required and training researchers in their use. As a staff member in the Bio-Imaging Resource Center (BIRC), will liaise between the research scientists, the existing microscopy and image analysis staff, and a new advanced image analyst who will implement deep learning-based approaches to analyze the light-sheet data. The instruments will be built in a dedicated new laboratory space housed on bedrock underneath the existing BIRC laboratory. Light sheet systems to be established will be pre-commercial systems such as the Lattice Light Sheet and MOSAIC systems, using designs kindly provided by the Janelia Research Campus. Additional responsibilities include implementing other pre-commercial systems in due course, as well as executing custom modifications of other BIRC microscopes as required.
Ph.D. or equivalent in optical engineering, physics, biophysics, or related field required. Experience in programming scientific instrumentation, teaching, and training a must. Must have a broad understanding of optical imaging techniques and the ability to build bespoke microscopes or other instruments from scratch. Must have a working knowledge of image and data acquisition, preferably with imaging biological samples. Must be enthusiastic, rigorous, motivated, have excellent interpersonal and communication skills. Must work well with a team in a service-oriented environment. Must be a self-starter with a proven ability to initiate collaborative projects and to introduce biologists to complex equipment. Must have the ability to establish close networks with colleagues nationwide involved in similar projects as this senior position will involve a significant degree of autonomy.
New York New York