"This work is made available in the hope it will be useful to researchers in biology who need to quickly get to grips with the main principles of image analysis."
Pete Bankhead's book works through the key concepts in image analysis in three parts (listed below). Along the way, you will be working through some clever examples using both his original art as well as standard fluorescence images.
PART I: INTRODUCING IMAGES, IMAGEJ & FIJI
PART II: PROCESSING FUNDAMENTALS
PART III: FLUORESCENCE IMAGES